Omics · study · 2026
Transcriptomic sequencing analysis of PPRV-infected goat endometrial epithelial cells and the critical role of the STING signaling pathway in PPRV infection
Listed in NCBI GEO
Peste des petits ruminants virus (PPRV) is the causative agent of peste des petits ruminants (PPR), a highly contagious disease in small ruminants that causes massive economic losses worldwide.
Description
Despite its impact, the mechanisms underlying PPRV infections remain elusive, hindering its control and prevention. This study examined the molecular mechanisms underlying PPRV infections by investigating the transcriptomic changes in PPRV-infected goat endometrial epithelial cells (EECs) and clarifying the regulatory role of the stimulator of interferon genes (STING) signaling pathway in PPRV replication.
A total of 1946 DEGs (1,078 up-regulated and 868 down-regulated) were identifie in PPRV-infected EECs. GO and KEGG enrichment analysis showed that the DEGs were κB and tumor necrosis factor signaling pathways. Western blot analysis showed that PPRV promotes STING and p-TBK1 expression.
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Specifically, overexpression of STING significantl enhanced PPRV replication, while the knockdown of STING by specific siRNA markedly reduced STING protein expression and significantly decreased the PPRV-N protein levels and viral titer (TCID50) in Vero CCL-81 and glomerular microvascular endothelial cells.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE343nnn/GSE343985 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE343985 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1514048 ncbi.nlm.nih.gov/bioproject/PRJNA1514048 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Cancer 65% · Disease 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE343985 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |