Transcriptome analysis reveals the potential mechanism of MAP34-B target to Pasteurella multocida Original data

It has been found that antimicrobial peptide MAP34-B exhibits strong antibacterial activity against Pasteurella multocida, although the specific mechanism of its antibacterial action remains unclear. To investigate the precise antibacterial mechanism of MAP34-B against Pasteurella multocida, Clinical strain HB03 of Pasteurella multocida, was conducted transcriptomic analysis on bacterial samples of strain HB03 treated with or without MAP34-B (47.4 µM) for 1 hour using RNA sequencing (RNA-seq).The results showed that, after treatment with MAP34-B, 281 differentially expressed genes were identified, including 161 upregulated genes and 120 downregulated genes.

KEGG pathway enrichment analysis revealed that the Ribosome pathway had the highest proportion of affected genes. After treatment with MAP34-B, the expression levels of genes rps2, rps3, rps9, rps16, rpl3, rpl9, rpl22, and rpl23, were upregulated, which may affect bacterial protein synthesis. Additionally, the expression levels of membrane-associated genes, such as SecE, SecG, lolB, and ompR, were also altered, disrupting the integrity of the bacterial cell membrane.