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Data · dataset · 2014

Marine microbial eukaryote transcriptomes

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Description

Overview This repository provides assemblies of a sub-set publicly available short read (Illumina 50bp PE) datasets of marine microbial eukaryotes. The raw data were generated by the Marine Microbial Eukaryote Transcriptome Sequencing Project (marinemicroeukaryotes.org/). This project provides its own set of assemblies (generated using the Abyss assembler).

Since our work-flows are geared towards assemblies generated by the Trinity assembler, raw data were obtained from the following website marinemicroeukaryotes.org/ and assembled using Trinity (trinityrnaseq.sourceforge.net/) For our purposes the raw data were directly assembled using Trinity (see README for version number) using the default settings. Several species/strains were assembled using all available Illumina libraries combined (see README).

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The identifiers of the raw data are provided and culture conditions of the organisms can be retrieved from marinemicroeukaryotes.org/ using the MMETSP ID (see README for list). If you use these data please cite Keeling, P.J. et al., 2014. The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): Illuminating the Functional Diversity of Eukaryotic Life in the Oceans through Transcriptome Sequencing.

PLoS Biology, 12:e1001889. Grabherr, M.G. et al., 2011. Full-length transcriptome assembly from RNA-Seq data without a reference genome.

Nature Biotechnology, 29:644-652. Funding NSF Dimensions of Biodiversity Grant DEB-1046075

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Stated by source
Biological sciences
Inferred from text
RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 11 field assertions
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DataCite10.6084/m9.figshare.115272713 d agoJSON v1
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