Biosystematic studies on the mountain plant Gentiana lutea L. reveal variability in reproductive traits among subspecies

Methods

We analysed morphological characters in 70 herbarium specimens. In five natural populations we counted pollen and ovule numbers, assessed reproductive output after pollination treatments and evaluated pre-dispersal predation. Results: Taxonomic traits previously indicated as diagnostic were not sufficient to discriminate among subspecies.

The pollen number and pollen:ovule (P:O) ratio varied strongly among subspecies; self-pollinated flowers produced a significantly lower number of seeds than open-pollinated flowers. Retention of empty fruits and high levels of pre-dispersal seed predation were observed in every case. Conclusions: The variation of P:O ratios among subspecies suggests different efficiency in pollen transfer.

The species is self-compatible, nevertheless all subspecies require pollen vectors to enhance cross pollination and viable seed production. Fruit retention may have evolved as a strategy to reduce predation, ensuring higher plant fitness.