Western blot raw data for “Rhein alleviates acute pancreatitis by modulating the gut microbiota–TMAO–TLR4/NF-κB/NLRP3 axis

This dataset contains the original, uncropped Western blot membranes supporting the study “Rhein alleviates acute pancreatitis by modulating the gut microbiota–TMAO–TLR4/NF-κB/NLRP3 axis.”The dataset includes raw immunoblot images from both in vivo pancreatic tissues and in vitro 266-6 acinar cell experiments, used to evaluate the regulatory effects of rhein on the TMAO–TLR4/NF-κB/NLRP3 inflammatory pathway.Experimental contents1. 266-6 pancreatic acinar cells – TLR4 inhibition validationGroups: AP, AP+TAK-242, AP+TMAO, AP+TMAO+TAK-242Purpose: To determine whether TLR4 mediates the pro-inflammatory effects of TMAO in acinar cells.Targets: TLR4, p-NF-κB p65, NF-κB p65, NLRP3, ACTIN.Corresponding excel: Figure 52. 266-6 pancreatic acinar cells – TMAO-related mechanismGroups: Control, AP, AP+TMAO, AP+TMAO+RheinPurpose: To verify the inhibitory effects of rhein on TMAO-induced activation of the TLR4/NF-κB/NLRP3 pathway.Targets: TLR4, p-NF-κB p65, NF-κB p65, NLRP3, Caspase-1, GSDMD-N, ACTIN.Corresponding excel: Figure 5(2) and Supplementary Figure S103.

Pancreatic tissue (in vivo)Groups: Control, AP,TMAO, Rhein.Purpose: To assess the inhibitory effects of rhein on the TMAO-mediated inflammatory signaling in acute pancreatitis.Targets: TLR4, phosphorylated NF-κB p65 (p-p65), NF-κB p65, NLRP3, ACTIN (loading control).Corresponding excel: Supplementary Figure S10.