Expression patterns of 10 key nitrogen transporter genes in STWs, their diploid parents and NTW under NN and LN conditions after 7 days of treatment.

This dataset contains raw quantitative fluorescence PCR detection data and calculated relative expression indices related to 10 key nitrogen transporter genes in synthetic tetraploid wheats (STWs), their diploid parents, and natural tetraploid wheat (NTW) under normal nitrogen (NN) or low nitrogen (LN) conditions, sampled at 7 days after LN treatment initiation (LN-7d). The relative expression levels of the 10 target genes were calculated using the 2^-ΔCt method with Actin as the internal reference gene.

The dataset also includes the relative expression fold changes of each gene under LN treatment compared with NN conditions across all tested genotypes.Statistical significance of expression differences was evaluated using two methods based on comparison types:For the effect of LN treatment on gene expression within the same genotype: Statistical significance was determined by two-tailed Student’s t-test, with significance levels marked as *P < 0.05, **P < 0.01, ***P < 0.001 in the associated visualization files.For differences in gene expression levels across different genotypes under the same LN treatment condition, as well as the relative expression fold changes of the 10 genes across genotypes: Significant differences were tested via one-way analysis of variance (ANOVA) followed by Tukey HSD tests.