Supplementary Datasets for Amino acid and codon usage explain amino acid misincorporation rates across the tree of life

Here, we analyzed thousands of publicly available mass spectrometry datasets to detect amino acid misincorporations and quantify error rates in 14 model organisms. We find that overall error rates and the patterns of codon to amino acid error rates correlate across species.

We estimate that on average 0.5-3% of protein molecules in a cell harbor a misincorporation, whereas this proportion can reach 10% for very long proteins. Highly expressed and very long proteins have lower error rates, indicating evolutionary selection on codon usage to reduce the cost of translation errors. While both codon-anticodon mispairing and tRNA mischarging contribute to misincorporations, we estimate that ~70% of misincorporation events are due to mispairing.