<p>Upstream Variability in IFNγ Signaling Amongst Keratinocytes from Asymptomatic Donors and Donors with Ulcerative Disease.</p>

Live cell images were acquired every 2 hours for 48 hours and normalized to measure GFP fluorescence. <b>(C)</b> The amount of IFNγ required to inhibit 50% of viral GFP expression was calculated for keratinocytes from both asymptomatic and ulcerative donors at 24 hours post infection. <b>(D)</b> Expression of IFNGR1 and STAT1 was measured by RT-qPCR in untreated keratinocytes from asymptomatic or ulcerative donors. <b>(E)</b> Representative images from a single donor illustrating the detection of pSTAT1 in the nucleus of keratinocytes treated with various doses of IFNγ for the specified time. <b>(F)</b> Representative images from each donor at 60 minutes following treatment with 1U of IFNγ highlight heterogeneity in nuclear pSTAT1 signal.

Yellow is nuclear DAPI staining, blue is membrane staining with wheat-germ agglutinin, and pink is pSTAT1. Scale bar represents 100µm <b>(E</b> and <b>F)</b>. <b>(G)</b> Normalized average nuclear fluorescence of pSTAT1 at specified times following treatment with specified doses of IFNγ, separated by donor cohort. Data was normalized using the asymptomatic donor pool (0% set by 0U IFNγ, 100% set by 100U IFNγ).