Effects of seabuckthorn juice on blood pressure and cardiac function in rats fed with high-salt diet-raw data

SD rats were randomly allocated into three groups: control group, high-salt diet group, and SBJ group. At 0, 1, 3, 5, and 7 weeks of the experiment, tail artery blood pressure was monitored using the tail-cuff method.

After eight weeks, the cardiac index was calculated, left ventricular catheterization was performed to measure hemodynamic changes in the left ventricle, and Sirius Red staining was utilized to observe myocardial fibrosis, expressions of the inflammation-related proteins NOD-like receptor pyrin domain-containing protein 3 (NLRP3), interleukin-1β (IL-1β) and cyclooxygenase-2 (COX-2) in the heart were examined using Western blot, ELISA was employed to detect myocardial injury markers including myoglobin (MYO), high-sensitivity cardiac troponin (hs-cTn), vascular endothelial injury markers like endothelin-1 (ET-1), nitric oxide (NO), vascular endothelial inflammation markers like soluble intercellular adhesion molecule-1 (sICAM-1), soluble vascular cell adhesion molecule (sVCAM-1), and inflammatory factors such as high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α), and IL-1β in serum.