Data for: Modification of the “Acute” method for calculating the final preferred temperatures as applied to Daphnia longispina (Crustacea: Cladocera)

However, for such conditions, the FPT should be calculated not at the point of the intersection of the preferred temperature (PT) line with the median, but over the range of values limited by the standard deviation lines of the PT crossing the median. We believe that this approach allows to calculate the correspondence of the preferred temperature and the temperature conditions in which the objects of study lived in the natural environment more accurately.

Using D. longispina as an example, it was shown that the “acute” method can be used to determine the FPT not only in animals acclimated to constant temperatures, but also in animals captured from natural habitats and placed in a gradient without preliminary acclimation. But in this case, the determination of the final preferred temperatures can not only be limited to the “acute” method in its classical formulation. To identify the full range of preferred temperatures, it is necessary to conduct an additional analysis of the graph used in calculation of the FPT.