Omics · study · 2026
Protective effect of energized structured water on bioenergetic function and oxidative stress in H9c2 cells: An explorative study
Listed in NCBI GEO
The energized structured water (ESW) concept supposes water can be imbued with energetic frequencies or vibrations, improving its quality and providing various health benefits.
Description
This study investigates the impact of ESW on cellular bioenergetic function and oxidative stress in H9c2 cardiomyocytes. H9c2 cells were pretreated with ESW or control water (CW) at equivalent dilutions.
Mitochondrial function was assessed using the Agilent Seahorse XF Pro Analyzer. Results demonstrated that ESW pretreatment significantly increased maximum and spare respiratory capacity in H9c2 cells. In addition, ESW treatment increased the glycolytic ATP production without affecting mitochondrial and total ATP production.
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ESW treatment enhanced cell viability and reduced ROS production in cells exposed to H2O2-induced oxidative stress. Based on differential gene expression analysis, 717 genes including Myh1, Akr1b8, Hmox1, Kcng1, Ugt1a6, Clu, Gaa, Ftl1, Hspb7, and Gba1 were up-regulated and 422 genes including Hist1h2an, Slfn4, Rpl22l1, Polr2k, Il1rl1, Ndufb1, Atp5mkl1 were down-regulated by ESW compared to the controls. Gene Ontology (GO) analysis indicated that ESW treatment significantly affects biological processes related to cellular stress response pathways.
These findings suggest that ESW treatment may enhance cellular bioenergetics and stress resistance in H9c2 cells, potentially through modulation of gene expression related to stress responses and energy metabolism in H9c2 cells.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE278nnn/GSE278403 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE278403 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1167193 ncbi.nlm.nih.gov/bioproject/PRJNA1167193 ↗
project · from NCBI GEO
- PubMed 40768905 pubmed.ncbi.nlm.nih.gov/40768905 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Rattus norvegicus
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE278403 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10116 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |