Omics · study · 2026
A STAT3 inhibitor Niclosamide Inhibits PRRSV replication
Listed in NCBI GEO
Porcine reproductive and respiratory syndrome (PRRS) caused by the PRRS virus (PRRSV) remains one of the most economically significant diseases in the global swine industry.
Description
Currently, there are no highly effective prevention and control methods. CD163 plays a crucial role in PRRSV entry, but direct manipulation of CD163 may cause potential risks due to its physiological functions.
To explore safer and more effective therapeutic approaches, we screened compounds targeting STAT3 that regulates CD163 expression. Among 45 commercially available STAT3 inhibitors, Niclosamide (NIC) and ML116 significantly reduced CD163 expression with minimal cytotoxicity. PRRSV infection assay further demonstrated that NIC effectively inhibited viral replication of both type I and type II PRRSV strains.
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Additionally, NIC synergized with our previously identified compound B7, allowing both drugs to be used at lower concentrations while maintaining strong antiviral activity. RNA-seq analysis of NIC-treated PAMs revealed broad modulation of innate immune pathways, including notable effects on the RIG-I-like receptor signaling pathway. These findings indicate that NIC is a promising anti PRRSV agent that functions by suppressing CD163 expression and modulating immune-related signaling pathways.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE322nnn/GSE322584 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322584 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1430589 ncbi.nlm.nih.gov/bioproject/PRJNA1430589 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Sus scrofa
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE322584 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9823 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |