Data from: A systems biology approach identifies B cell maturation antigen (BCMA) as biomarker reflecting oral vaccine induced IgA antibody responses in humans

In this study, RNA-Seq was performed on peripheral blood mononuclear cells (PBMCs) isolated from six volunteers of a oral cholera Dukoral® vaccine study performed by Mottram et al., 2020.(10.1016/j.vaccine.2019.10.050 (doi.org/10.1016/j.vaccine.2019.10.050) ). The raw FASQ and indexed aligned BAM files described here were taken from four strong and two non IgA antibody responders of this Dukoral® vaccine study and are part of another study described by Mottram et al (2021) (Frontiers in Immunology, accepted for publication).

Total RNA-Seq on these PMBCs was performed by GeneCore SU, University of Gothenburg, Sahlgrenska Academy, Gothenburg, Sweden (cgg.gu.se/genecore-su), using the Illumina Nextseq 500 platform (Illumina, San Diego, CA, USA). cDNA libraries were prepared from total RNA samples (≤1500ng), using the TruSeq Stranded Total RNA Sample Preparation Kit with Ribo-zero Gold (Illumina Inc). Each library was paired-end sequenced (2×75 bp) using the Illumina Nextseq500 Kit High Output V2.