Data · dataset · 2024
Revision-Effect of the Stability of the Poly(gamma-Glutamic Acid)-ACP Dispersion System on Biomimetic Mineralization of Type I Collagen.
Listed in ScienceDB
Description
Objective: To explore the relationship between the stability of poly(gamma-glutamic acid) (γ-PGA) dispersion systems with γ-PGA of different molecular weights (MWs) and concentrations and type I collagen mineralization.Methods: γ-PGA was used as a noncollagenous protein (NCP) analogue to regulate the stability of supersaturated γ-PGA-stabilized amorphous calcium phosphate (PGA-ACP) solutions by changing the γ-PGA MW (2, 10, 100, 200 and 500 kDa) and concentration (400, 500 and 600 μg/mL).
Then, the optical density (OD) at 72 h was measured to determine the PGA-ACP solution stability. Recombinant type I collagen films were mineralized in different PGA-ACP solutions for 3 d and observed via transmission electron microscopy (TEM) to confirm the occurrence of intrafibrillar mineralization. The collagen scaffolds were mineralized for 7 d and observed via scanning electron microscopy (SEM) to determine the collagen mineralization pattern and degree.
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X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and thermogravimetry (TG) were used to analyse the mineralized collagen scaffold composition.Results: The PGA-ACP solutions with γ-PGA of different MWs and concentrations had different stabilities and type I collagen mineralization. Except for the 100 kDa group, which neither stabilized the supersaturated calcium phosphate solution nor induced intrafibrillar mineralization, the groups stabilized the solutions for at least 10 h and induced different intrafibrillar mineralization patterns and degrees.
Conclusion: In our system, the PGA-ACP solution stability and occurrence of intrafibrillar mineralization are directly correlated. Thus, we suspect that the same correspondence exists in other biomimetic mineralization systems and that a relatively stable supersaturated calcium phosphate solution may be a necessary condition for intrafibrillar mineralization.
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Where it is published
- DOI doi.org/10.57760/sciencedb.18002 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Microscopy 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.18002 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:microscopy | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |