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Data · dataset · 2025

The RNA-seq data of taplt muant root for the article "Functional characterization of the effects of gene TaPLT8 on iron distribution in different wheat organs"

Listed in ScienceDB

Wheat seeds (cultivar ‘Fielder’ and transgenic lines) for hydroponic experiments were surface sterilized for 30 min in 10% H2O2, followed by five rinses with distilled water, and germinated on wet filter paper in darkness at 16 ℃ for four days.

Description

The seedings were then placed in Hoagland’s nutrient solution with the following composition: 4 mmol L−1 Ca(NO3)2, 6 mmol L−1 KNO3, 1 mmol L−1 NH4H2PO4, 2 mmol L−1 MgSO4, 10 mmol L−1 H3BO3, 1.8 mmol L−1 MnSO4, 5 mmol L−1 ZnSO4, 0.31 mmol L−1 CuSO4, 0.2 mmol L−1 NaMoO4, and 50 mmol L−1 EDTA-Fe(III), pH = 6.0, and the solutions were renewed every 3 days.

The plants were grown in a phytotron with a photoperiod of 16 h light (22 °C) and 8 h darkness (18 °C) for 4 weeks. The datasets generated are also available at the National Center for Biotechnology Information (NCBI) SRA repository under accession number PRJNA953366.

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Where it is published

Catalogue records · 1

Topics

Inferred from text
Agricultural biotechnology 75% · RNA sequencing 65%
Provenance · 1 source records, 12 field assertions
SourceKeyLast seenRaw
ScienceDB10.57760/sciencedb.j00210.0004211 d agoJSON v1
FieldAssertionExtractorEvidence
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