Data · dataset · 2026
Molecular cloning and bioactivity assessment of a bioactive peptide QUB-1509 from the skin secretion of the Asian frog Hylarana guentheri
Listed in ZivaHub and Deakin Research Online and DMU Figshare and UCL Research Data Repository — shown once because both records carry DOI 10.17034/32629932.v1
The abuse of antibiotics has resulted in the increase of drug-resistant bacteria and as numerous people have now died from super-bacterial infections worldwide, the quest for and development of novel antibiotics is urgent and significant.
Description
Temporins are among the smallest peptides that have been discovered to disrupt the bacterial membrane directly which makes it difficult for bacteria to develop resistance.<br><br>In this study, the antimicrobial peptide QUB-1509, which was isolated from the skin secretion of the Asian frog, Hylarana guentheri, and belongs to the temporin family, was focused upon.
A cDNA library was constructed by using the isolated mRNA from the skin secretion and ‘shotgun’ cloning was employed as an interrogation technique. After the mature sequence of the peptide was deduced from cloned precursor-encoding cDNA, solid phase peptide synthesis (SPPS) was employed to obtain the crude peptide. Reversed-Phase High Performance Liquid Chromatography (RP-HPLC) and Matrix-Assisted Laser Desorption Ionization, Time-of-Flight (MALDI-TOF) mass spectrometry were used to purify and identify QUB-1509.
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Bioassay of QUB-1509 showed that it possessed a broad-spectrum antibacterial activity against S. aureus (MIC=32 μM, MBC=64 μM), E. coli (MIC=128 μM, MBC=256 μM), C. albicans (MIC=128 μM, MBC=256 μM). QUB-1509 also showed cytotoxicity towards both HCT116 and U251MG human cancer cell lines. In the haemolysis assay, QUB-1509 showed low cytotoxicity towards horse red blood cells with no more than 15% haemolysis at 512 μM. These results have laid the foundation for the development of QUB-1509 as a potential antibacterial therapeutic.
Links
Where it is published
- DOI doi.org/10.17034/32629932.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Life Sciences · Life Sciences · Life Sciences · Life Sciences
- Inferred from text
- Agricultural biotechnology 70% · Cancer 75% · Mass spectrometry 75%
Provenance · 4 source records, 15 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32629932 | 12 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32629932 | 12 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32629932 | 12 d ago | JSON v1 |
| UCL Research Data Repository | oai:figshare.com:article/32629932 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].anzsrc:group:3001 | enrichment · zivahub uct ac za | taxonomy-embedding@1.1.0 | title+keywords+description (70%) |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |