Uncovering the hidden within shipping containers: Molecular biosurveillance confirms a pathway for introducing multiple regulated and invasive species

The origin of the eDNA detected from each species was linked to their native distribution and country of origin, except for Lymantria dispar. Additionally, four physical specimens were collected from shipping container debris and DNA barcoded, identifying three non-regulated species (two arthropods and one fungus). Altogether, these results demonstrate the importance of integrating molecular identification into current toolkits for the biosurveillance of invasive alien species.

Additionally, it reaffirms international shipping containers as a pathway for multiple invasive aliens and regulated species introduction in Canada. It also highlights the need to establish regular and effective molecular biosurveillance at the Canadian border to avoid new or recurrent invasions.