ranscriptome sequencing

Three technical replicates were set during sequencing to control experimental errors, and data reliability was verified via quality control procedures.3 in wound repair after endoscopic submucosal dissection (ESD).Wound tissues of rats and pigs after ESD were selected as research objects, with a control group (treated with normal saline only) and a CA treatment group set up. Samples were collected from wound tissues at key postoperative time points (e.g., 1d, 3d, 7d after surgery).

Total RNA was extracted from samples using the Trizol method, and after verifying RNA quality via Nanodrop and agarose gel electrophoresis, transcriptome sequencing (PE150) was performed using the Illumina NovaSeq 6000 platform. Subsequent quality control was conducted on raw sequencing data (removing low-quality reads and adapter sequences), and the filtered data were aligned to the corresponding reference genomes (Rnor_6.0 for rats, Sscrofa11.1 for pigs) to finally obtain differentially expressed gene matrices, FPKM/TPM expression values, etc.The dataset contains transcriptome sequencing data of samples, with data formats mainly including fastq (raw sequencing data) and txt (gene expression matrix), and no missing values.

Three technical replicates were set during sequencing to control experimental errors, and data reliability was verified via quality control procedures.