Data · dataset · 2026
Identification and functional study of a bioactive peptide, QUB-2323, from the skin secretion of the orange-legged monkey tree frog, <i>Phyllomedusa azurea</i>
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32641902.v1
The widespread use of antibiotics has contributed significantly to the emergence of bacterial resistance.
Description
Antimicrobial peptides (AMPs) have emerged as promising antibacterial drug candidates due to their broad-spectrum of activity and low potential for resistance development. In this study, the precursor cDNA encoding an AMP, named QUB-2323, was isolated from the skin secretion of the orange-legged monkey tree frog, Phyllomedusa azurea, using shotgun cloning.
Sequence analysis identified QUB-2323 as a member of the Dermaseptin family of AMPs, with the mature peptide sequence of GLWSTIKNVGKEAAIAAGKAALGAL-amide. <br><br>A series of experiments was conducted to evaluate the biological activity of QUB-2323. The peptide displayed broad-spectrum antimicrobial activity, with minimum inhibitory concentrations (MICs) of 1 µM against Staphylococcus aureus (NCTC 10788) and Candida albicans (NCYC 1467), and 0.5 µM against Escherichia coli (NCTC 10418).
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QUB-2323 also exhibited anti-proliferative activity, with an IC50 value of 6.44 µM against the lung cancer cell line, NCI-H838. However, the peptide showed notable cytotoxicity: a haemolytic concentration (HC₅₀) of approximately 128 µM against horse erythrocytes and an IC₅₀ of 2.377 µM against normal human lung fibroblasts (MRC-5). Comparison of these values revealed therapeutic indices of 128–256 for antibacterial activity but a low selectivity index of 0.37 for anti-proliferative activity, indicating higher toxicity toward normal fibroblasts than cancer cells.
Collectively, these findings suggest that QUB-2323 holds promise as a lead candidate for developing novel antibacterial and anticancer therapies, although its therapeutic applications may be limited by cytotoxicity.<br><br><i>Thesis is embargoed until 31 December 2030.</i>
Links
Where it is published
- DOI doi.org/10.17034/32641902.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- Cancer 75%
Provenance · 3 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32641902 | 10 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32641902 | 10 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32641902 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |