Omics · study · 2026
Chemically Induced Alanine Auxotrophy of Mycobacterium tuberculosis
Listed in NCBI GEO
Utilizing a novel computational chemogenomics method to discover new antitubercular compounds, a hit compound designated TI-374 was identified with a minimal inhibitory concentration of 540 nM against M. tuberculosis H37Rv.
Description
To investigate the mechanism of action, transcriptional profiling of treated cultures was conducted by RNA-seq. Combined with computational chemogenomic target prediction, metabolomic analysis of treated cultures, nutritional suppression experiments, activity in biochemical assays against purified protein, and whole-genome sequencing of resistant mutants, this transcriptomic data is consistent with a novel mechanism of action whereby TI-374 inhibits the alanine aminotransferase annotated as aspC.
This activity interferes with alanine biosynthesis, which is required for growth of the pathogen.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE267nnn/GSE267532 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE267532 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1111794 ncbi.nlm.nih.gov/bioproject/PRJNA1111794 ↗
project · from NCBI GEO
- PubMed 42466413 pubmed.ncbi.nlm.nih.gov/42466413 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Genome sequencing 65% · RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE267532 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:genome-sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |