Omics · study · 2026
Na-dependent gene regulation in kidney and heart
Listed in NCBI GEO
We examined Na-dependent changes in gene expression in the renal papilla and heart ventricle of rats.
Description
Animals were conditioned with control or low-Na diets for 1 week. RNA isolated from kidneys or hearts was analyzed by RNAseq.
In the renal papilla, notable genes upregulated in response to low dietary Na included alpha, betta, and gamma subunits of ENaC, alpha and beta subunits of the Na/K-ATPase, and the transcription factor Zbtb16. Increases in mRNA for the ENaC were confirmed using qPCR and were strongly dependent on location within the kidney with papilla > outer medulla > cortex. Differences in ENaC protein expression exhibited a similar dependence on location.
Read the rest (2 more)
The effects of dietary Na+ on ENaC expression, as well as that of Zbtb16, in the papillae were not mimicked by treatment with aldosterone, indicating that other factors contribute to the adaptation to Na+ deprivation. In the heart, a smaller number of genes were affected by dietary Na+. Effects clearly related to Na+ balance included downregulation of transcripts for the natriuretic peptides ANP and BNP.
Enrichment analyses indicated that low dietary Na led to upregulation of many Gene Ontology (GO) gene sets related to growth and development in the kidney samples, but downregulation of such gene sets in the heart. Our results illustrate the profound site- and tissue-specific effects of changes in dietary Na+ on renal and cardiac gene expression and regulation of salt balance.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330800 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330800 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1465655 ncbi.nlm.nih.gov/bioproject/PRJNA1465655 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Rattus norvegicus
- From keywords
- Life Sciences
- Inferred from text
- Heart 75% · RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE330800 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10116 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |