Omics · study · 2026
Transcriptional profiling of CRP-V5 and K52Q-V5 Vibrio cholerae C6706 grown in M9 minimal medium with maltose or trehalose as sole carbon source
Listed in NCBI GEO
RNA-seq was performed to compare the transcriptomes of Vibrio cholerae C6706 strains expressing V5-tagged wild-type CRP (CRP-V5) or the acetylation-mimicking mutant K52Q-V5 during growth in M9 minimal medium supplemented with 0.8% maltose or 0.8% trehalose as the sole carbon source.
Description
Overnight cultures grown in LB were washed and resuspended in the respective M9 media, then cultivated for approximately 3 hours to mid-exponential phase.
Total RNA was extracted and subjected to stranded total RNA sequencing with ribosomal RNA depletion. This dataset complements ChIP-seq and RNA-seq data previously deposited under GSE310654 by examining CRP-dependent transcriptional responses under carbon source-specific conditions.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE324nnn/GSE324475 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE324475 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1435215 ncbi.nlm.nih.gov/bioproject/PRJNA1435215 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Evolutionary biology 68% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE324475 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3104 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (68%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |