AFM images and AFM force curves characterizing S. aureus lipid membrane upon interaction with antimicrobial peptidomimetic AMC-109, Mass Spectroscopy data of S. aureus lipid extracts

Each TXT file contains 2 data sets corresponding to the approach and retract force-indentation curve one after another, organized in 2 columns showing "Vertical Tip Position" and "Vertical Deflection". Mass Spectroscopy data of S. aureus lipid extracts in their raw format gathered using an Ultra-high performance liquid chromatography-mass spectroscopy instrument consisting of Accela1250 UHPLC system (Thermo Fisher Scientific) coupled to a Thermo Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific) equipped with an ESO ion-source in negative ionization mode.

Detailed description of the individual folders: Folder "Figure 1": AFM raw images of S. aureus lipid membrane before and after step by step addition of antimicrobial peptidomimetic AMC-109. Folder "Figure S2": AFM raw images of S. aureus lipid membrane that is expanding in time after treatment with antimicrobial peptidomimetic AMC-109. Folder "Figure S3": AFM raw images of S. aureus lipid membrane before and after addition of antimicrobial peptidomimetic AMC-109 in a concentration above its minimal inhibitory concentration .

Folder "Figure S5": AFM force-indentation curves gathered on S. aureus lipid membranes without any treatment and after treatment with subcritical concentrations of the antimicrobial AMC-109. The force-indentation curves were used to characterize the mechanical properties of the membrane upon the antibiotic treatment. The folder "Figure S5" contains three subfolders that contain individual force-indentation curves measured on S. aureus lipid membranes exposed to no AMC-109 (0_ug-ml_AMC-109), 0.5 ug/ml AMC-109 (0p5_ug-ml_AMC-109), and 1 ug/ml AMC-109 (1_ug-ml_AMC-109).

Folder "Figure S7": AFM raw images of AMC-109 layer forming on the mica support. Mica was exposed to the PBS buffer with 2 ug/ml AMC-109, this layer was scratched by contact imaging with the AFM tip. The AFM images show the recovery of the AMC-109 layer on mica.

Folder "Figure S14": AFM raw images of the S. aureus lipid membrane before and after the exposure to 0.023 vol% of DMSO. Folder "Table 1 and Figure S1": Mass Spectroscopy data of S. aureus lipid extracts in their raw format.