<p>Data PLOS.</p>

The methodology is centered on the nanosecond pulsed laser ablation of a synthetic hydroxyapatite (HAp) target, physically modified through the incorporation of metallic copper filaments, which serve as a localized dopant source during the deposition process.

This configuration enables the controlled co-deposition of hydroxyapatite and copper species, facilitating the formation of uniform thin films with potential bioactive properties. The proposed protocol comprises the following key elements: • <b>Preparation of Targets:</b> A comprehensive, stepwise procedure for the synthesis of hydroxyapatite powder and the subsequent fabrication of mechanically robust ceramic targets, including a novel approach for embedding copper filaments to ensure effective doping during laser ablation. • <b>Deposition Parameters:</b> Detailed specifications of the PAPLD system, encompassing laser characteristics (wavelength, fluence, repetition rate), target-to-substrate distance, and chamber vacuum conditions.