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Table · dataset · 2026

A CRISPR-SHERLOCK-based biosensor detecting mcyE and mcyB microcystin synthase gene RNA transcripts as an early sensing system for microcystin production

Listed in HKU DataHub and figshare and Loughborough Research Repository — shown once because both records carry DOI 10.6084/m9.figshare.33324522.v1

<p dir="ltr">Raw plate-reader fluorescence data from three independent CRISPR-Cas13a (SHERLOCK) experiments testing crRNA performance against microcystin synthetase gene transcripts.

Description

Experiments were run on 3 August, 17 August and 18 August 2026 by the BOSLab iGEM 2025 team.<br><br>Seven crRNAs were tested: bot1, bot2 and bot3 (targeting mcyB), eot1, eot2 and eot3 (targeting mcyE), and 16sg (16S rRNA control guide). Each crRNA was run in triplicate under three conditions: normal (target RNA present), no guide, and mismatch.

Control wells included no-Cas, probe-only and RNase conditions. Fluorescence was read at 5-minute intervals through a 60-minute reaction endpoint. The platemap image shows the well layout used in all three runs.<br><br>Files: <br>- 8_03_plate_reader_results.xlsx: raw plate-reader output, experiment 1 <br>- 8_17_plate_reading_results.xlsx: raw plate-reader output, experiment 2 <br>- 8_18_plate_reading_results.xlsx: raw plate-reader output, experiment 3 <br>- platemap.png: plate layout and well assignments</p>

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Astronomy & Astrophysics · Chemistry · Chemistry · Computer Science & AI · Computer Science & AI · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Economics & Finance · Economics & Finance · Engineering · Engineering · Humanities · Humanities · Life Sciences · Life Sciences · Life Sciences · Materials Science · Mathematics & Statistics · Medicine & Health · Medicine & Health · Ocean & Atmospheric Science · Psychology & Behavioral Science · Social Science · Social Science · Synthetic biology · Synthetic biology · Synthetic biology
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Image 75%
Provenance · 3 source records, 34 field assertions
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HKU DataHuboai:figshare.com:article/3332452210 d agoJSON v1
figshareoai:figshare.com:article/333245229 d agoJSON v1
Loughborough Research Repositoryoai:figshare.com:article/333245229 d agoJSON v1
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