Microflora Danica: Bacterial 16S rRNA genes (UMIs)

This project contains the near full-length UMI 16S rRNA gene data belonging to the Microflora Danica project. The Microflora Danica project encompasses over 10,000 samples from across Denmark, taken between 2019 and 2023 with the goal to cover as much of the microbial diversity in the investigated habitats as possible. For the bacterial and eukaryotic operon data see doi.org/10.15468/yr5rmw and doi.org/10.15468/ea7jvq, respectively.

The unique molecular identifiers (UMIs) approach relies on the use of molecular nucleotide template tagging to achieve high-accuracy single-molecule consensus calling on the Oxford Nanopore sequencing platform. The addition of UMIs to both ends of the template enables the bioinformatic identification and removal of chimeras formed during PCR. In the project the UMI 16S rRNA gene sequences was used together with near full-length 16S rRNA gene sequences to construct the Microflora Danica 16S rRNA gene reference database (zenodo.org/records/12605769) and to investigate the 16S rRNA gene sequence diversity and novelty of species representative sequences.