Data · study · 2026
NGS of viral ds cDNA reads in plants
Listed in NCBI GEO
We previously demonstrated that two naturally occurring single-nucleotide mutations affecting distinct amino acids in the HCPro of a potyviral chimera independently increased virulence by enhancing either silencing suppression or proteolytic activity.
Description
To examine how beneficial variants arising during infection compete with the wild-type and with each other, we co-expressed pairs of these variants in Nicotiana benthamiana leaves.
We then evaluated their coexistence and interactions within host cells, as well as the impact of abiotic (elevated temperature) and biotic (silencing-impaired host) factors on infection dynamics. Outcomes were assessed in both local tissues at 5 days post-inoculation (dpi) and systemic tissues at 29 dpi.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327379 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327379 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1450631 ncbi.nlm.nih.gov/bioproject/PRJNA1450631 ↗
project · from NCBI GEO
- PubMed 42611933 pubmed.ncbi.nlm.nih.gov/42611933 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Other
- From keywords
- Life Sciences
- Inferred from text
- Agricultural biotechnology 73% · Sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE327379 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3001 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (73%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |