Anticancer and antioxidant Holuthuria edulis

Sea cucumbers are extracted using three methods, namely maceration, soxhletation and ultrasonication. The data displayed is related to the calculation of toxicity data for the BSLT method, antioxidants and anticancer MCF-7 cells.

The BSLT method toxicity calculation is as follows, the calculation of toxicity (IC50) was carried out using probit analysis. Number of dead larvae % death = ------------------------ x 100% Number of test larvae The IC50 was then calculated A blank – A sample % inhibition = -------------------- x 100% A blank The IC50 was then calculated: The absorbance of treated cells – absorbance of control media % cell death = -------------------------------------------------------------------- x 100% The absorbance of control cell – Absorbance of control media