Protein Folding and Embryogenesis in Antarctic Fishes: A Comparative Approach to Environmental Stress

The specific aims of this work are: 1) to determine the capacity of the chaperonin complex of notothenioid fishes to assist protein folding at temperatures between -4 and +20°C; and 2) to evaluate the genetic responses of notothenioid embryos, measured as global differential gene transcription, to temperature challenge, with -1.9°C as the "normal" control and +4 and +10°C as high temperature insults. The physiology of embryonic development of marine stenotherms under future climate change scenarios is an important but understudied problem.

This project will provide valuable insights into the capacity of Antarctic fish embryos to acclimatize and adapt to plausible climate change scenarios by examining multiple levels of biological organization, from the biochemical to the organismal. The results should also be broadly applicable to understanding the impact of global warming on marine biota worldwide. The research will also introduce graduate and undergraduate students to state-of-the-art biochemical, cellular, and molecular-biological research relevant to ecological and environmental issues of the Antarctic marine ecosystem.