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Data · dataset · 2026

<p>Identification and characterization of MHC-II immunopeptidome of <i>Francisella</i>-infected BMDCs.</p>

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<p>(A) Histogram showing the dynamic range of the identified immunopeptidome and the relationship between endogenous mouse (left axis) and <i>Francisella</i> (right axis) peptides.

Description

MS signal intensities of peptides identified at q-value <0.01 from a sample of BMDCs infected with LVSΔdsbA are shown. (B) MS/MS spectrum of the <i>Francisella</i> peptide KINTTNAPQAIGSYEQ with annotation of b-ions (red, including water, ammonia loss) and y-ions (blue, including water loss), obtained for the doubly protonated precursor ion.

(C) Extracted ion chromatograms for the peptide identified in (B) from analyses of uninfected, LVS-infected, and LVSΔdsbA-infected BMDCs. (D) Bacterial peptides identified in BMDCs infected with LVS or LVSΔdsbA. Proportions of identifications assigned from peptide-spectrum matches (PSMs) and from peptide feature matches (matched) between LVS and LVSΔdsbA are shown.

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Identifications assigned from peptide-to-spectrum matches and from match-between-runs comparison are shown. (E) Sequence length distribution of identified endogenous murine (left axis, n = 5,545) and bacterial (right axis, n = 57) peptides. (F) Mouse peptides (n = 5,441, 9-30 aa in length) and <i>Francisella</i> peptides (n = 57, 11-23 aa in length) were classified as strong, weak, and non-binding based on predicted binding to the I-A<sup>b</sup> molecule. %rank thresholds of <1%, < 5%, and ≥5% were used.

(G) Peptide binding motif determined for bacterial peptides (n = 57) and for (H) endogenous mouse peptides (n = 5,441) using the GibbsCluster-2.0 tool. (I) The 57 <i>Francisella</i> MHC-II peptides clustered into 24 epitope regions originating from 23 different protein antigens. (J) The sequences of the identified epitope regions of <i>F. tularensis</i> LVS were evaluated for conservation in other strains of this bacterium using BLASTP.

The proportions of identical, homologous, or those not found in the given strain are shown.</p>

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Biochemistry and cell biology · Cancer · Earth & Environmental Science · Immunology · Infectious diseases · Life Sciences · Medicine & Health · Microbiology
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ZivaHuboai:figshare.com:article/3402691912 d agoJSON v1
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