Data · dataset · 2026
Identification and functional evaluation of a novel peptide QUB 2537, isolated from the skin secretion of<i> Phyllomedusa burmeisteri</i>
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32638548.v1
Since the discovery of penicillin, bacterial infection is no longer an incurable disease.
Description
With theemergence of bacterial resistance, the varieties of antibiotics are greatly reduced, resulting inincreased difficulty in clinical treatment. Therefore, new antibiotic candidates are urgent todiscover and apply in clinical treatment.
This study aims to identify a novel antimicrobial peptideform the skin secretion of Phyllomedusa burmeisteri to evaluate its bioactivities and toxicity. Inorder to obtain the cDNA sequence, the “shot gun” cloning method was utilized. After that, thepeptide was chemically synthesised followed by cleavage and purification.
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Also, biologicalactivity testing was then carried out. Additionally, the characteristics of QUB 2537 which is fromthe Dermaseptin family were predicted and designed as a truncated analogue. To assess thebiological activity of peptides, antimicrobial, anticancer, and haemolysis experiments werecarried out.
It showed that QUB 2537 may suppress the growth of the three testedmicroorganisms at concentrations from 2~64 μM and yet only caused less than 50% haemolysisat 512 μM. In addition, it inhibited the proliferation of human lung cancer cell line H838 at 10-4M. Based on its antimicrobial activity, it has the potential to become a substitute for antibiotics.<br><br><i>Thesis embargoed until 31st December 2028</i>
Links
Where it is published
- DOI doi.org/10.17034/32638548.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
Provenance · 3 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32638548 | 13 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32638548 | 13 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32638548 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[disease].local:disease:disease | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |