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Table · dataset · 2026

Supplementary Data – Alpinia chinensis essential oils

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<p dir="ltr">Herbicide resistance is eroding the reliability of conventional grass-weed control in direct-seeded rice and increases the need for chemically diverse, biorational leads.

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We evaluated essential oils (EOs) obtained separately from the rhizome, leaf, fruit, stem and flower of Alpinia chinensis (Retz.) Roscoe. GC–MS identified 74 volatile constituents representing 96.07–97.63% of each oil and revealed pronounced organ-specific chemical differentiation.

Phytotoxicity was quantified at 0–1.60 µL mL⁻¹ against two cultivated rice genotypes (OM18 and RVT), weedy rice, Echinochloa crus-galli, E. colona and Leptochloa chinensis. Factorial inference based on raw dish-mean seedling lengths showed highly significant effects of dose, EO source organ and their interaction for every weed response (all P < 0.001; partial η² = 0.812–1.000). L. chinensis was exceptionally sensitive: leaf EO caused 100% shoot and root inhibition at 1.60 µL mL⁻¹, while ED₅₀ estimates within the tested range were 0.189–0.412 µL mL⁻¹ for shoots and 0.094–0.433 µL mL⁻¹ for roots.

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Echinochloa responses were strong but oil- and species-dependent, whereas weedy-rice shoots showed organ-specific hormesis. Across the full dose range, leaf EO maximized weed bioactivity, but flower EO produced the widest weed–rice selectivity gap (18.46 percentage points; 95% bootstrap CI 17.91–19.00) and the highest weed/rice ratio (1.96; 95% CI 1.91–2.02). These results provide organ-resolved evidence that A. chinensis contains chemically distinct phytotoxic mixtures with contrasting potency and crop selectivity, prioritizing flower EO for selective formulation and leaf EO for bioactivity-guided fractionation.</p>

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Crop and pasture production 70%
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