Raw Data - Hypoxic Preconditioning Promotes Survivals of Human Adipocyte Mesenchymal Stem Cell via Expression of Prosurvival and Proangiogenic Biomarkers

This research was conducted through 4 stages. First, isolation of h-AMSCs culture from adipose tissue of patient (human). Second, the characterization of h-AMSCs from adipose tissue by phenotype (flowcytometry) through CD44+, CD90+ and CD45-expression before being pre-conditioned for hypoxic treatment.

Third, the hypoxic preconditioning in h-AMSCs culture (in vitro) was performed with an oxygen concentration of 1% for 24, 48 and 72 hours. Fourth, observation of survival from h-AMSCs culture was tested on the role of CD44+, VEGF, SCF, OCT-4, BCL2, HSP27 with Flowcytometry and apoptotic inhibition by Tunnel Assay method. Results .

The result of regression test showed that time difference had an effect on VEGF expression ( p <0.001; β =-0.482) and hypoxia condition also influenced VEGF expression ( p <0.001; β =0.774). The result of path analysis showed that SCF had effect on OCT-4 expression ( p <0.001; β =0.985). The regression test results showed that time effects on HSP27 expression ( p <0.001; β =0.398) and hypoxia precondition also affects HSP27 expression ( p <0.001; β =0.847).

Pathway analysis showed that BCL2 expression inhibited apoptosis ( p =0.030; β =-0.442) and HSP27 expression also inhibited apoptosis ( p <0,001; β =-0.487). Conclusion . Hypoxic preconditioning of h-AMSC culture has proven to increase the expression of VEGF, SCF, OCT-4, and BCL2 and HSP27.

This study demonstrated and explained the existence of a new mechanism of increased h-AMSC survival in cultures with hypoxic preconditioning (O2 1%) via VEGF, SCF, OCT-4, BCL2, and HSP 27.