Cell‑type‑specific gene expression profiling in Stevia rebaudiana leaves by single‑nucleus RNA sequencing

In this study, we used single‑nucleus RNA sequencing (snRNA‑seq) to resolve the expression landscape of SG‑biosynthetic genes across individual cell types in stevia leaves. We identified distinct nuclear clusters corresponding to different cell types such as mesophyll, epidermis, trichomes and vascular bundle sheath.

Notably, UGT76G‑family and UGT91D4 sugar‑transferase genes showed high expression in mesophyll and epidermal nuclei, suggesting spatial division of SG production within leaf tissues.