Codes for Higher actin contractility and cargo-loaded clathrin pits create midplane-peaked apico-basal tension profiles in HeLa cells

Folder “OT_image_analysis” contains two folders, the first “OT_image_analysis”, used for the force measurements by image analysis, and the other is “Stage_Run_Labview_code”, used to move the stage at a particular velocity of 0.5 μm/sec away from the trapped bead for tether pulling experiments. The “OT_image_analysis” module contains the required functions and code to determine the centroid of the bead, which is optically trapped for force measurements.

The main code here is “trap_all_files.m” used to find the centroid of the bead by fitting the periphery to a circle. Folder “transferrin_clathrin_analysis” has a code “tf_clathrin_cortex_wc_analysis.m” to analyse transferrin and clathrin colocalization at the cortex per image stack. After generating it, the data was collected by using the code “data_collect_all.m” for further analysis.