汉防己甲素对放射性肺损伤的保护作用及机制研究

Results: Compared with the control group, the coefficient (1.27 ± 0.05 vs 1.55 ± 0.20), histology score (2.00 ± 1.27 vs 6.00 ± 1.27) and the protein expression levels of MPO (1.00 ± 0.21 vs 1.34 ± 0.20), F4/80 (1.00 ± 0.13 vs 1.28 ± 0.11), IL1β (1.00 ± 0.18 vs 1.71 ± 0.14), IL6 (1.00 ± 0.17 vs 1.62 ± 0.31), CD86 (1.00 ± 0.29 vs 1.62 ± 0.36), NLRP3 (1.00 ± 0.24 vs 1.54 ± 0.46), and Caspase1 (1.00 ± 0.20 vs 1.66 ± 0.30) in the irradiation group were increased, whereas the protein expression of CD163 (1.00 ± 0.21 vs 0.59 ± 0.07) was decreased.

Compared with the irradiation group, the coefficient (1.36 ± 0.07), histology score (3.17 ± 1.33) and the protein expression levels of MPO (1.02 ± 0.10), F4/80 (0.94 ± 0.23), IL1β (0.97 ± 0.56), IL6 (1.01 ± 0.10), CD86 (1.07 ± 0.34), NLRP3 (1.02 ± 0.25), and Caspase1 (0.74 ± 0.37) in the irradiation plus tetrandrine group were decreased, whereas the protein expression of CD163 (0.99 ± 0.25) was increased.

Conclusions: Tetrandrine can alleviate RILI, possibly by regulating the NLRP3/Caspase1 pathway to promote M2 polarization of macrophages and inhibit inflammatory responses.