Dynamic binding of the bacterial chaperone Trigger factor to translating ribosomes in Escherichia coli

Further, binding events are interrupted only by transient excursions to a freely diffusing state (ca 40 ms), suggesting a highly dynamic binding and unbinding cycle of TF in vivo. We also show that TF competes with SRP for RNC binding, and in doing so, tunes the binding selectivity of SRP. The data and corresponding analyses have been divided into folders corresponding to different experimental conditions.

Each experimental condition folder contains raw microscopy images ("RawData") with three phase contrast images ("A_Phase"), one brightfield image ("B_Bf"), and a stack of fluorescence images ("C_553", "C_546", or "C_638", depending on what laser was used). The "Tracking_EXP_YY_YYYY" folder contains single-particle tracking analysis of the corresponding raw data, and the "HMM_analysis" folder contains HMM-based diffusion analysis of the single-particle trajectories.

The "Tools" folder contains all software for data analysis, and "Figures_and_tables_scripts" contains scripts for generating figures and tables.