Investigation of the Anti-Lung Cancer Activity and Mechanism of Umbilical Cord Blood Stem Cell-Derived NK Cell Exosomes

Uptake by cells after exosome treatment was checked with confocal microscopy. A549 xenograft tumors were made in nude mice, and UCB-NK-EXO was given through the tail vein. The tumor growth happens and the liver & kidney in serum was checked for functions tests.

I used western blot methodology to find out if there was a related-apoptosis signaling molecule in the tumor squads.Results UCB-NK-EXO greatly inhibited the proliferation of A549 cells (P <0.01), and promoted the apoptosis of the cells (P<0.001). It was also successfully internalized by the cells. The UCB-NK-EXO group had a much stronger anti-tumor effect than the DOX group in the in vivo experiments and also had a better effect on survival time p<0.001.

UCB-NK-EXO treatment has no bodyweight loss and no hepatorenal toxicity of above Western blot results show cytotoxic protein like Granzyme B & Perforin exist in UCB-NK-EXO and Tumor tissue has very high expression of Apoptotic proteins like Caspase related proteins.Conclusion UCB-NK-EXO effectively inhibits the growth of A549 lung cancer both in vitro and in vivo. The mechanism involves the delivery of cytotoxic proteins to tumor cells, activation of the Caspase-dependent apoptosis pathway, and subsequent induction of tumor cell apoptosis.

UCB-NK-EXO demonstrates superior antitumor efficacy and a more favorable safety profile compared to the conventional chemotherapeutic agent doxorubicin.