Data for the article "The lack of avoidance of total dissolved gas supersaturation in juvenile rainbow trout"

As there is no mechanism by which fish can detect and avoid TDG supersaturation in shallow water, we hypothesize that rainbow trout do not directly detect TDG supersaturation. Most previous studies have tested TDG avoidance in groups of fish, which may confound responses because many fishes, such as juvenile rainbow trout, display territorial behavior.

We placed rainbow trout individually into a flume and allowed them to swim freely between two channels for 6 min; one channel contained water at 145% TDG and the other contained air-equilibrated water. We then switched treatments between channels and tested fish in the same way for an additional 6 min. We quantified the duration spent by fish in each channel during the last 2 min of each 6 min exposure.