Raw smFRET data, analysis scripts and cryo-EM maps for: The substrate-binding domains of the osmoregulatory ABC importer OpuA physically interact

The dataset also contains the cyro-EM maps and the low-pass-filtered cryo-EM maps. Bacteria utilize various strategies to prevent internal dehydration during hypertonic stress.

A common approach to countering the effects of the stress is to import compatible solutes such as glycine betaine, leading to simultaneous passive water fluxes following the osmotic gradient. OpuA from Lactococcus lactis is a type I ABC-importer that uses two substrate-binding domains (SBDs) to capture extracellular glycine betaine and deliver the substrate to the transmembrane domains for subsequent transport. OpuA senses osmotic stress via changes in the internal ionic strength and is furthermore regulated by the 2nd messenger cyclic-di-AMP.