Omics · study · 2026
Intranasal ΔNS1-86 Live Attenuated Vaccines Confer Sterilizing Immunity to Mammalian-Adapted H5N1
Listed in NCBI GEO
Influenza A viruses continue to evolve across avian and mammalian hosts, highlighting the need for vaccines that elicit broad and durable immunity.
Description
Here, we developed a modular live-attenuated influenza vaccine (LAIV) platform based on a short NS1 truncation (ΔNS1-86) engineered to express heterologous HA1 antigens through a multibasic-cleavage and 2A-peptide system. ΔNS1-86 LAIVs replicated in an attenuated manner while maintaining stable antigen expression.
In mice, a single intranasal dose induced strong neutralizing antibodies and robust cross-reactive IFN-γ–producing T cell responses against divergent H5N1 clades and H9N2 lineages. In ferrets, the ΔNS1-86 LAIV provided sterilizing protection against a highly mammalian-adapted North American clade 2.3.4.4b H5N1 virus, completely blocking viral shedding and systemic dissemination—efficacy that surpassed a two-dose inactivated vaccine despite similar neutralization titers.
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The platform was adaptable to multiple HA/NA backbones and compatible with DIVA diagnostics. These findings establish ΔNS1-86 LAIVs as versatile candidates for broad influenza protection and pandemic preparedness.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE326nnn/GSE326254 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE326254 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1442995 ncbi.nlm.nih.gov/bioproject/PRJNA1442995 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
Provenance · 1 source records, 6 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE326254 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |