Mytilus galloprovincialis 4 tissues x 5 salinities x 2 times

Cellular damage – Biomarkers of oxidative or membrane damage. Neurotoxicity – Enzyme related to neuronal function and inhibition by contaminants. Biochemical biomarkers SDH – Succinate dehydrogenase activity (mitochondrial metabolism) ETS – Electron transport system activity (overall metabolic capacity) PROT – Soluble protein content GLY – Glycogen content SOD – Superoxide dismutase activity GPx – Glutathione peroxidase activity GR – Glutathione reductase activity GSTs – Glutathione S-transferases activity TAC – Total antioxidant capacity CbEs – Carboxylesterases activity LPO – Lipid peroxidation (oxidative damage indicator) AChE – Acetylcholinesterase activity (neuromuscular function marker) Column descriptions Functional Group: Biochemical category of the biomarker (Metabolism, Antioxidant, CellDamage, or Neurotoxicity).

Tissue: Organ analyzed (DG, G, MU, MA). Biomarker: Specific biochemical parameter measured. Salinity / Salinity (vs 30): Salinity level (20, 25, 30, 35, 40) compared with reference salinity (30).

Time: Exposure time (14 or 28 days). mean_14 / mean_28: Mean value at 14 or 28 days. sd_14 / sd_28: Standard deviation corresponding to each mean. mean_30 / mean_comp: Mean values for salinity 30 and for the compared salinity, respectively. sd_30 / sd_comp: Standard deviations corresponding to each mean. Time p / Salinity p: p-value for the statistical comparison. Test Used: Statistical test applied (t-test or Wilcoxon-Mann–Whitney).