X-ray diffraction data using ice-binding protein (LeIBP) crystal

LeIBP exhibits low amino acid sequence similarity to other antifreeze proteins with known protein structures. Recently, we developed an expression system allowing high-level production and efficient purification of recombinant pLeIBP.

Furthermore, crystallization and preliminary X-ray crystallographic analysis of the ice-binding protein were performed. To investigate the antifreeze mechanism of LeIBP, we have carried out structural studies. As the first step toward its structural elucidation, we report the results of preliminary X-ray crystallographic experiments with LeIBP.