Data · dataset · 2025
Sequencing of Recombinant Baculovirus Vectors - p6.9 rBEV
Listed in Borealis
2nd passage (P2) recombinant baculovirus vectors having a reporter green fluorescent protein (mAzami Green) under the control of the p6.9 promoter.
Description
The recombinant viruses were amplified in Spodoptera frugiperda (Sf9) cells at a low multiplicity of infection (MOI), ~0.1, and harvested 72 hours post infection (hpi). The baculovirus genomic DNAs (gDNAs) from the propagated P2 p6.9GFP rBEV stock or P2 p6.9GFP_sgRNA_gp64+131 rBEV stock were extracted and sequenced via shotgun sequencing or tiled-amplicon sequencing, respectively.
Control and CRISPR-Cas9 targeted baculovirus genomes, upon plasmid or rBEV-based delivery of sgRNA into Sf9-Cas9 cells, were extracted and sequenced via tiled-amplicon sequencing.
Links
Where it is published
- Dataverse dataset page borealisdata.ca/dataset.xhtml?persistentId=doi%3A10.5683%2FSP3%2FFIBEX4 ↗
landing page · from borealisdata ca
- DOI doi.org/10.5683/sp3/fibex4 ↗
DOI / persistent id · from borealisdata ca
Catalogue records · 1
- Dataverse API borealisdata.ca/api/datasets/:persistentId/?persistentId=doi%3A10.5683%2FSP3%2… ↗
metadata API · from borealisdata ca
Topics
- Stated by source
- Engineering · Medicine, Health and Life Sciences
- From keywords
- Engineering · Life Sciences · Medicine & Health
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Borealis | doi:10.5683/SP3/FIBEX4 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].dataverse_subject:engineering | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].dataverse_subject:medicine-health-and-life-sciences | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].local:field:engineering | mapping · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].local:field:life-sciences | mapping · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].local:field:medicine-health | mapping · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[modality].local:modality:sequencing | enrichment · borealisdata ca | keyword-concept-rules@1.0.0 | title+description (75%) |
| created_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| description | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /description |
| publication_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| title | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /name |
| updated_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| version_label | source · borealisdata ca | connector:borealisdata_ca@1.0.0 |