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[Chemotaxis of V. alginolyticus towards Synechococcus Cells] - (Collaborative Research: Viral induced chemotaxis mediating cross-trophic microbial interactions and carbon flux)

Listed in Biological and Chemical Oceanography Data Management Office (BCO-DMO) ERDDAP

Description

This data set summarises the chemotactic response of model heterotrophic bacteria, Vibrio alginolyticus towards phage-infected cyanobacteria Synechococcus cells/exudates respectively. Phage treatments are fully described in: doi.org/10.1038/s43705-022-00169-6. Six microfluidic experiments were conducted each on different days: three with phage infection and three control, uninfected experiments.

Both uninfected and phage-infected experiments were performed identically, with the substitution of phage addition for an equivalent volume of SN media in the uninfected experiments. All infection experiments were performed within a week using the same phage stock, with Synechococcus WH8102 as the host. Non-motile host cells were loaded into a microfluidic chamber with the model heterotrophic bacteria V. alginolyticus.

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Analysis of the cell spatial distribution over time revealed a strong sustained accumulation of chemotactic bacteria towards phage-infected cyanobacteria, and no measurable accumulation in the control condition. This provides the first direct experimental evidence of chemotaxis of heterotrophic bacteria towards cyanobacteria.

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Provenance · 1 source records, 4 field assertions
SourceKeyLast seenRaw
Biological and Chemical Oceanography Data Management Office (BCO-DMO) ERDDAPbcodmo_dataset_913619_v111 d agoJSON v1
FieldAssertionExtractorEvidence
access_levelsource · erddap bco dmo orgconnector:erddap_bco_dmo_org@1.0.0
concepts[field].local:field:ocean-atmosphericmapping · erddap bco dmo orgconnector:erddap_bco_dmo_org@1.0.0
descriptionsource · erddap bco dmo orgconnector:erddap_bco_dmo_org@1.0.0/summary
titlesource · erddap bco dmo orgconnector:erddap_bco_dmo_org@1.0.0/title