Data Sheet 1_Scutellarein promotes diabetic wound repair and preserves fibroblast calcium-mitochondrial homeostasis.pdf

Scutellarein (SCU), a structurally defined plant-derived flavonoid metabolite, has documented antioxidant and anti-inflammatory activities; however, its role and the mechanistically relevant pathways engaged in diabetic wound repair, particularly those involving calcium-mitochondrial homeostasis and purinergic signaling, remain incompletely defined.</p>Methods<p>We administered local SCU in a streptozotocin-induced diabetic mouse cutaneous wound model and, in H2O2-injured L929 fibroblasts, assessed scratch closure, survival, redox balance, mitochondrial morphology and function, and calcium overload.

We then used RNA sequencing (RNA-seq) and adenosine triphosphate (ATP) challenge/reversal experiments to examine purinergic calcium signaling. These analyses identified P2rx1—the gene encoding P2RX1 (P2X purinoceptor 1), an ATP-gated cation channel that mediates rapid calcium entry—as a candidate purinergic signal rather than a validated target.</p>Results<p>SCU accelerated wound closure and increased re-epithelialization, Masson-positive collagen deposition, proliferative α-SMA-positive wound cells, and angiogenic indices in diabetic wounds.