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Data · dataset · 2022

Transcriptome data of Thitarodes xiaojinensis larvae infected with entomopathogenic fungi

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Sixth-instar Thitarodes xiaojinensis larvae were infected with the blastospores of Ophiocordyceps sinensis (Os), Cordyceps militarist (Cm), or Beauveria bassiana (Bb).

Description

Larvae injected with Ringer’s buffer (RS, 8.05 g NaCl, 0.42 g KCl, and 0.18 g CaCl2 per liter) were used as the control. Each larva was injected with 2 µL of Ringer’s buffer or blastospore suspension in Ringer’s buffer (1.0 × 107 cells/mL) using glass capillaries.

Each treatment included 12 larvae. According to the manufacturer’s instructions, total RNA from the fat body of control and fungus-infected larvae was extracted at 72 hpi using TRIzol reagent (Invitrogen, USA). RNA yields were quantified using a NanoDrop 2000 spectrophotometer.

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RNA integrity was confirmed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system. A cDNA library was constructed using the total RNA and sequenced with 150 bp paired-end reads on an Illumina NovaSeq platform. Three biological replicates were used for each treatment.

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ScienceDB10.11922/sciencedb.o00019.0001111 d agoJSON v1
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concepts[field].local:field:earth-environmentalmapping · scidb cnconnector:scidb_cn@1.0.0
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concepts[field].local:field:humanitiesmapping · scidb cnconnector:scidb_cn@1.0.0
concepts[field].local:field:life-sciencesmapping · scidb cnconnector:scidb_cn@1.0.0
concepts[field].local:field:social-sciencemapping · scidb cnconnector:scidb_cn@1.0.0
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