Data · dataset · 2021

Molecular assays to detect the presence and viability of Phytophthora ramorum and Grosmannia clavigera

Listed in Borealis and Agri-environmental Research Data Dataverse — shown once because both records carry DOI 10.5683/sp2/zsawxj

Description

Abstract

Protocols for the RNA and DNA extractions, cDNA synthesis and qPCR and RT-qPCR for detecting and assessing viability of the Phytophthora ramorum and Grosmannia clavigera tree pathogens.

Read the rest (2 more)

Methods

Fifty to 80 mg of wood scrapings were collected at each inoculation point from eight logs of four conifer species and used for the simultaneous extractions of gDNA and mRNA. Using the diluted RNA, cDNA, synthesis was performed using the QuantiTect Reverse Transcription Kit (QIAGEN).

Topics

Stated by source: Other · Other
From keywords: Chemistry · Computer Science & AI · Earth & Environmental Science · Economics & Finance · Engineering · Humanities · Life Sciences · Mathematics & Statistics · Medicine & Health · Ocean & Atmospheric Science · Social Science
Inferred from text: Plant biology 69%

Provenance · 2 source records, 20 field assertions

Source	Key	Last seen	Raw
Borealis	doi:10.5683/SP2/ZSAWXJ	11 d ago	JSON v1
Agri-environmental Research Data Dataverse	doi:10.5683/SP2/ZSAWXJ	10 d ago	JSON v1

Field	Assertion	Extractor	Evidence
concepts[field].anzsrc:group:3108	enrichment · borealisdata ca	taxonomy-embedding@1.0.0	title+keywords+description (69%)
concepts[field].dataverse_subject:other	source · borealisdata ca	connector:borealisdata_ca@1.0.0	/subjects
concepts[field].dataverse_subject:other	source · borealisdata ca dataverse	connector:borealisdata_ca_dataverse@1.0.0	/subjects
concepts[field].local:field:chemistry	mapping · borealisdata ca dataverse	connector:borealisdata_ca_dataverse@1.0.0	/subjects
concepts[field].local:field:computer-science-ai	mapping · borealisdata ca dataverse	connector:borealisdata_ca_dataverse@1.0.0	/subjects
concepts[field].local:field:earth-environmental	mapping · borealisdata ca dataverse	connector:borealisdata_ca_dataverse@1.0.0	/subjects
concepts[field].local:field:economics-finance	mapping · borealisdata ca dataverse	connector:borealisdata_ca_dataverse@1.0.0	/subjects
concepts[field].local:field:engineering	mapping · borealisdata ca dataverse	connector:borealisdata_ca_dataverse@1.0.0	/subjects
concepts[field].local:field:humanities	mapping · borealisdata ca dataverse	connector:borealisdata_ca_dataverse@1.0.0	/subjects
concepts[field].local:field:life-sciences	mapping · borealisdata ca dataverse	connector:borealisdata_ca_dataverse@1.0.0	/subjects
concepts[field].local:field:mathematics-statistics	mapping · borealisdata ca dataverse	connector:borealisdata_ca_dataverse@1.0.0	/subjects
concepts[field].local:field:medicine-health	mapping · borealisdata ca dataverse	connector:borealisdata_ca_dataverse@1.0.0	/subjects
concepts[field].local:field:ocean-atmospheric	mapping · borealisdata ca dataverse	connector:borealisdata_ca_dataverse@1.0.0	/subjects
concepts[field].local:field:social-science	mapping · borealisdata ca dataverse	connector:borealisdata_ca_dataverse@1.0.0	/subjects
created_date	source · borealisdata ca	connector:borealisdata_ca@1.0.0
description	source · borealisdata ca	connector:borealisdata_ca@1.0.0	/description
publication_date	source · borealisdata ca	connector:borealisdata_ca@1.0.0
title	source · borealisdata ca	connector:borealisdata_ca@1.0.0	/name
updated_date	source · borealisdata ca	connector:borealisdata_ca@1.0.0
version_label	source · borealisdata ca	connector:borealisdata_ca@1.0.0