The antitumor efficacy of Ganoderma lucidum spore oil in hepatoma H22-bearing mice depends on modulating immunity and inhibiting eicosanoid metabolism pathway in tumor cells

T and B lymphocyte proliferation, delayed hypersensitivity, NK cell killing activity, macrophage phagocytotic activity, cytokine levels and CD69 molecule expression were detected to estimate immune function. Flow cytometry and Pro-DIA quantitative proteomics analysis were performed to investigate the potential mechanism of GLSO in tumor inhibition, which was verified by WB and RT-PCR. Thirty-eight compounds including triterpenoids, fatty acids and esters, were identified from GLSO. GLSO treatment prevented tumor occurrence and inhibited tumor growth because of the smaller initial and final tumor volumes and lower Ki67 expression.

Treatment with GLSO promoted a strong immune response in hepatoma H22-bearing mice and improved tumor immune microenvironment. Proteomics analysis showed that GLSO inhibited eicosanoid metabolism pathway, WB and RT-PCR re-check these results. In conclusion, these findings support that GLSO prevents the occurrence of tumor depending on its immunomodulatory effects and we first report that GLSO restricts tumor growth by inhibiting eicosanoid metabolism pathway.