Omics · study · 2026
Engineered Senescence-Targeting Apoptotic Extracellular Vesicles-Laden Injectable Hydrogel Microspheres Mitigating Nucleus Pulposus Cell Ferroptosis against Intervertebral Disc Degeneration
Listed in NCBI GEO
As the rapid growth of aging population, intervertebral disc degeneration (IVDD) becomes a prevalent degenerative disorder in clinical practice.
Description
Ferroptosis-induced senescence in nucleus pulposus cells (NPCs) represents a primary pathological mechanism underlying IVDD. Strategies for reducing ferroptosis to delay NPC senescence hold prospects for therapeutic advances.
According to previously reported proteomic profiles of ApoEVs, we noticed that most key ferroptosis-regulating proteins were identified in the ApoEVs proteome, including a marked upregulation of GPX4. In this study, we developed injectable hydrogel microspheres integrating with senescent NPC-targeting apoptotic extracellular vesicles (ApoEVs) as GPX4 delivery vehicle and anti-inflammatory diclofenac sodium (DFS) for IVDD treatment.
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The effect of ApoEVs on reducing NPC ferroptosis and reversing NPC senescence-related metabolic dysfunction and damage was reinforced through modification of reactive oxygen species responsive and senescent NPC-targeting peptide. In vivo experiments and single cell RNA sequencing analysis elucidated that the senescence-targeted system significantly inhibited ferroptosis and inflammatory pathways, and preventedthe transformation of inflammatory NPC phenotypes, thereby offering opportunitiesfor rescuing senescent NPCs from ferroptosis and mitigating IVDD progression.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE312nnn/GSE312472 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE312472 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1373573 ncbi.nlm.nih.gov/bioproject/PRJNA1373573 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Rattus norvegicus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE312472 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10116 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |